Cell Painting is a powerful high-content imaging assay that captures rich morphological profiles of cells. Pooled Cell Painting, where image-based sequencing enables disambiguation of perturbations, enables massive scaling of the assay, but processing its data at such scale remains challenging. The Cimini Lab at the Broad Institute has been at the forefront of developing and applying Cell Painting, building tools like CellProfiler that handle everything from classical image analysis to complex workflows like pooled genetic perturbation screens. In this webinar, they share how adopting Nextflow and Seqera Platform through Seqera's Professional Services team, transformed how their pipelines are developed, deployed, and run at scale.